海洋Penicillium sp. FJ—1中一个抗真菌活性的新橙酮苷(1)
[摘要]红树植物内生真菌的代谢物中存在很多新的活性化合物。通过对红树植物海榄雌中一株内生真菌Penicillium sp. FJ-1的研究，利用硅胶柱色谱方法分离得到了一个新橙酮苷(Z)-7，4′-dimethoxy-6-hydroxy-aurone-4-O-β-glucopyranoside(1)。通过波谱分析方法确定其化学结构。化合物1具有显著的抗白色念珠菌活性，强度优于氟康唑，与两性霉素B相当，并且能够浓度依赖性抑制磷脂酶分泌。
[关键词]海洋来源真菌;橙酮苷;海榄雌;抗真菌活性
doi：10.4268/cjcmm20150621
[Received date]2014-11-04
[Corresponding author]*SONG Yan-xia， Tel：(0633) 2291925， E-mail： yanxiasongyx@163.com
, http://www.100md.com
Marine-derived fungi have been proved as a rich source of active secondary metabolites with unique structures and diversified biological activities[1]. Over the last decade， many new metabolites have been isolated and identified from marine-derived fungi， which has been the focus of discovery for new pharmacological products[2]. Mangrove plants are growing in subtropical and tropical intertidal habitats[3]. Many chemical constituents， such as anthraquinones， terpenoids and seiricuprolides were isolated from this plant or mangrove-derived fungus， and exhibited anti-inflammatory， antimicrobial and antiproliferative activities[4-6]. Glycosides， flavonoids and terpenoids are the main constituents in Avicennia marina[7-10]. Preliminary study showed that the EtOH extract from endophytic fungus Penicillium sp. FJ-1 of A. marina exerted inhibitory effect against Candida sp. Further investigation on the metabolites from the culture broth of this fungus resulted in the isolation of a new aurone glycoside (Z)-7，4′-dimethoxy-6-hydroxy-aurone-4-O-β-glucopyranoside (1) (Fig.1) by column chromatography and recrystallization methods. Herein we report the isolation， identification and antifungal activity of this compound.
, 百拇医药
1Experimental
1.1General experimental procedures
The UV spectrum was obtained using a Shimadzu UV-2401-A spectrophotometer with PhotoMultiplier Tube (Shimadzu， Japan). The NMR spectra were recorded on a Bruker AV-500 spectrometer (Bruker， Swiss)， using TMS as an internal standard. The HR-ESI-MS spectra were measured with an API QSTAR Pulsar mass Spectrometer (Bruker， Swiss). Column chromatography was performed with silica gel (200-300 mesh， Qingdao Haiyang Chemical Co.， Ltd， Qingdao， China). TLC was carried out on precoated silica gel GF-254 plates (Merck， Germany)， and spots were detected with 10% H2SO4 in alcohol followed by heating.
, http://www.100md.com
1.2Plant material
The plant of A. marina was collected in Zhangpu county， Fujian province， China， in Aug. 2013， and authenticated by Prof. Shang-Jun Yang， Institute of Materia Medica， Shandong Academy of Medical Sciences. A voucher specimen (No. 20130815) was deposited at the department of chemistry of natural product， institute of materia medica， Shandong academy of medical sciences., 百拇医药(宋艳霞 马强 李捷)
[关键词]海洋来源真菌;橙酮苷;海榄雌;抗真菌活性
doi：10.4268/cjcmm20150621
[Received date]2014-11-04
[Corresponding author]*SONG Yan-xia， Tel：(0633) 2291925， E-mail： yanxiasongyx@163.com
, http://www.100md.com
Marine-derived fungi have been proved as a rich source of active secondary metabolites with unique structures and diversified biological activities[1]. Over the last decade， many new metabolites have been isolated and identified from marine-derived fungi， which has been the focus of discovery for new pharmacological products[2]. Mangrove plants are growing in subtropical and tropical intertidal habitats[3]. Many chemical constituents， such as anthraquinones， terpenoids and seiricuprolides were isolated from this plant or mangrove-derived fungus， and exhibited anti-inflammatory， antimicrobial and antiproliferative activities[4-6]. Glycosides， flavonoids and terpenoids are the main constituents in Avicennia marina[7-10]. Preliminary study showed that the EtOH extract from endophytic fungus Penicillium sp. FJ-1 of A. marina exerted inhibitory effect against Candida sp. Further investigation on the metabolites from the culture broth of this fungus resulted in the isolation of a new aurone glycoside (Z)-7，4′-dimethoxy-6-hydroxy-aurone-4-O-β-glucopyranoside (1) (Fig.1) by column chromatography and recrystallization methods. Herein we report the isolation， identification and antifungal activity of this compound.
, 百拇医药
1Experimental
1.1General experimental procedures
The UV spectrum was obtained using a Shimadzu UV-2401-A spectrophotometer with PhotoMultiplier Tube (Shimadzu， Japan). The NMR spectra were recorded on a Bruker AV-500 spectrometer (Bruker， Swiss)， using TMS as an internal standard. The HR-ESI-MS spectra were measured with an API QSTAR Pulsar mass Spectrometer (Bruker， Swiss). Column chromatography was performed with silica gel (200-300 mesh， Qingdao Haiyang Chemical Co.， Ltd， Qingdao， China). TLC was carried out on precoated silica gel GF-254 plates (Merck， Germany)， and spots were detected with 10% H2SO4 in alcohol followed by heating.
, http://www.100md.com
1.2Plant material
The plant of A. marina was collected in Zhangpu county， Fujian province， China， in Aug. 2013， and authenticated by Prof. Shang-Jun Yang， Institute of Materia Medica， Shandong Academy of Medical Sciences. A voucher specimen (No. 20130815) was deposited at the department of chemistry of natural product， institute of materia medica， Shandong academy of medical sciences., 百拇医药(宋艳霞 马强 李捷)